Identification of membrane protein interactomes is a key issue to better understand how these molecules carry out their functions. However, protein-protein interactions using conventional interaction assays are particularly challenging for integral membrane proteins, because of their hydrophobic nature. Here we describe the membrane yeast two-hybrid (MbY2H) system, a powerful tool for identifying the interactors of membrane and membrane-associated proteins.
CITATION STYLE
Voisin, M., Vanrobays, E., & Tatout, C. (2018). Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System. In Methods in Molecular Biology (Vol. 1840, pp. 221–235). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8691-0_16
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