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Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains

by Lia Rosane Rodrigues, Bianca de Camargo Forte, Maria Helena Bodanese-Zanettini
Brazilian Archives of Biology and Technology ()

Abstract

Protoplasts were isolated from cotton microspores by hydrolysing the microspore wall with snailase and driselase; the snailase was superior to the driselase and the optimum concentration was 0.5%. Sucrose was used as an osmotic regulator, the optimum concentration being 20%. The protoplasts obtained by this method were cultured in K3 medium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre. The first cell divisions were observed after 3-4 days and the second after 10 days. No further cell division was observed, indicating that the culture conditions require further improvement.

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