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Isolation and Culture of Soybean (Glycine max L. Merrill) Microspores and Pollen Grains

by Bianca de Camargo Forte1 Lia Rosane Rodrigues1, Maria Helena Bodanese-Zanettini1
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY ()

Abstract

Protoplasts were isolated from cotton microspores by hydrolysing the\nmicrospore wall with snailase and driselase; the snailase was superior\nto the driselase and the optimum concentration was 0.5%. Sucrose\nwas used as an osmotic regulator, the optimum concentration being\n20%. The protoplasts obtained by this method were cultured in K3\nmedium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre.\nThe first cell divisions were observed after 3-4 days and the second\nafter 10 days. No further cell division was observed, indicating\nthat the culture conditions require further improvement.

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