Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains
- ISSN: 15168913
- DOI: 10.1590/S1516-89132006000500002
Protoplasts were isolated from cotton microspores by hydrolysing the microspore wall with snailase and driselase; the snailase was superior to the driselase and the optimum concentration was 0.5%. Sucrose was used as an osmotic regulator, the optimum concentration being 20%. The protoplasts obtained by this method were cultured in K3 medium containing 0.5 mg 2,4-D, 1.0 mg 2iP and 0.5 mg kinetin/litre. The first cell divisions were observed after 3-4 days and the second after 10 days. No further cell division was observed, indicating that the culture conditions require further improvement.