Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation procedures, we include extensive details in growing donor plants and caring for transgenic plants in the greenhouse.
CITATION STYLE
Frame, B. R., Paque, T., & Wang, K. (2006). Maize (Zea mays L.). Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1201/b10736-10
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