The dynamic co- and post-translational modification (PTM) of proteins, O-linked β- D -N-acetylglucosamine modification (O-GlcNAcylation) of serine/threonine residues is critical in many cellular processes, contributing to multiple physiological and pathological events. The term “O-GlcNAcome” refers to not only the complete set of proteins that undergo O-GlcNAcylation but also the O-GlcNAc status at individual residues, as well as the dynamics of O-GlcNAcylation in response to various stimuli. O-GlcNAcomic analyses have been a challenge for many years. In this chapter, we describe a recently developed approach for the identification and quantification of O-GlcNAc proteins/peptides from complex samples.
CITATION STYLE
Ma, J., & Hart, G. W. (2016). Mass spectrometry-based quantitative O-GlcNAcomic analysis. In Methods in Molecular Biology (Vol. 1410, pp. 91–103). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3524-6_6
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