Single nucleotide polymorphisms (SNPs) of matrix metalloproteinase3 (MMP3) promoter in the development and progression of gastric cancer of whole stomach has never been investigated in any population. We conducted a hospital-based case-control study to explore the MMP3 SNPs and their haplotypes with the risk of gastric cancer for the first time in eastern Indian population. A total of 218 gastric cancer patients and 175 healthy controls were genotyped for MMP3-1612 5A/6A (rs3025058) by PCR-RFLP and rechecked 10% by DNA sequencing. MMP3-707 A/G (rs522616) and MMP3-375C/G (rs617819) were genotyped by DNA sequencing among 209 patients and 154 controls. MMP3-1612 5A6A genotype (P=0.026, odds ratio (OR)=1.756, confidence interval (CI)=1.070-2.883), combined 5A5A and 5A6A genotype (P=0.015, OR=1.791, CI=1.122-2.858) and 5A allele (P=0.002, OR=1.75, CI=1.21-2.53) and; MMP3-707 GG genotype (P=<0.0001; OR=9.612; 95% CI=3.403-27.147), combined GG and AG genotype (P=0.001, OR=2.201, CI=1.385-3.498) and G allele (P=<0.0001, OR=2.189, CI=1.582-3.033) conferred significant risk for gastric cancer development. Also, tobacco addicted individuals with combined 5A5A and 5A6A genotype (P=0.005, OR=2.952, CI=1.377-6.327) at -1612 position of MMP3 promoter displayed a higher risk to gastric cancer development. The genotypic combinations of all three MMP3 promoter polymorphisms and their haplotypes with increasing risk allele in a dose-dependent manner showed a potential risk for developing gastric cancer. The analyses suggested that the MMP3-707 G/G and MMP3-1612 5A/6A polymorphisms are potential independent predictors of gastric cancer risk development. © 2011 Wiley Periodicals, Inc.
CITATION STYLE
Dey, S., Stalin, S., Gupta, A., Saha, D., Kesh, K., & Swarnakar, S. (2012). Matrix metalloproteinase3 gene promoter polymorphisms and their haplotypes are associated with gastric cancer risk in eastern Indian population. Molecular Carcinogenesis, 51(SUPPL. 1). https://doi.org/10.1002/mc.21837
Mendeley helps you to discover research relevant for your work.