Membrane Molecules Mobile even after Chemical Fixation

  • SUZUKI K
  • TANAKA K
  • KUSUMI A
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Abstract

Fixation of cells and tissues is the critical first step for histochemical or cytochemical investigations 1,2 . Recent efforts to visualize lipid rafts by immunofluorescence and immunoelectron microscopy 3 , particularly in nonstimulated cells, have yielded varied results, raising a question about the efficacy of chemical cross-linking fixation protocols for block - ing the lateral diffusion of membrane molecules and thus their antibody-induced clustering 3,4 . In a literature search we did not find any systematic investigation of this issue. Here we investi - gated whether the lateral diffusion of both raft-associated and non–raft-associated molecules is blocked upon chemical cross- linking and treatment with cold metha - nol in human T24 cells. We used single fluorescent molecule tracking, which allowed us to directly observe the varia - tion in molecular immobilization after fixation

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SUZUKI, K. G. N., TANAKA, K. A. K., & KUSUMI, A. (2011). Membrane Molecules Mobile even after Chemical Fixation. Seibutsu Butsuri, 51(5), 226–227. https://doi.org/10.2142/biophys.51.226

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