Tobacco rattle virus (TRV-K) was first identified in a symptomatic Gladiolus plant cultivated in Korea. We analyzed the TRV-K genome and compared its phylogeny with other TRV isolates. After constructing of a full-length genomic RNA2 strand clone, a complete sequence was generated from several overlapping clones. The cloned genome was 3261 bases in length, identical to TRV-K, and had three open reading frames. TRV-K had the highest sequence identity with the American isolate TRV-ORY. Sequence analysis of the RNA2 genome showed that TRV-K contains an intact 2a, 2b, and 2c coding sequence and an RNA1-related 3′ terminus, which is typical of TRV RNA2. Phylogenetic analysis revealed that TRV-K is in the same cluster as the American isolates and another Korean isolate, TRV-SK; however, it was in a different cluster than the European isolates.
CITATION STYLE
Kim, Y. J., Lim, M. S., Kim, S. M., Ryu, K. H., & Choi, S. H. (2015). Molecular characterization of the tobacco rattle virus RNA2 genome isolated from Gladiolus. Acta Biologica Hungarica, 66(2), 222–230. https://doi.org/10.1556/018.66.2015.2.8
Mendeley helps you to discover research relevant for your work.