Illegitimate recombination (IR) is a process that takes place far more often than homologous recombination and is characterized by the recombination between non-homologous or short homologous sequences. The consequences of IR frequently emerge after the introduction of DNA in cell lines because it more frequently integrates in non- homologous than in homologous regions of the host genome. As a result, unexpected truncated or elongated products may be found. By not discarding those products as transfection artifacts, but by studying how they are generated, it might elucidate a possible molecular mechanism of IR. Here we review the current literature describing different mechanisms by which non-homologous DNA recombination can be induced.
CITATION STYLE
van Rijk, A., & Bloemendal, H. (2003). Molecular mechanisms of exon shuffling: illegitimate recombination (pp. 245–249). https://doi.org/10.1007/978-94-010-0229-5_13
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