Background: The conjunctiva contains a specialized population of lymphocytes that reside in the epithelium, named intraepithelial lymphocytes (IEL). Methodology/Principal Findings: Here we characterized the IEL population prior to and after experimental desiccating stress (DS) for 5 or 10 days (DS5, DS10) and evaluated the effect of NK depletion on DS. The frequency of IELs in normal murine conjunctiva was CD3+CD103+ (~22%), CD3+γδ+ (~9.6%), CD3+NK+ (2%), CD3-NK+ (~4.4%), CD3+CD8α (~0.9%), and CD4 (~0.6%). Systemic depletion of NK cells prior and during DS led to a decrease in the frequency of total and activated DCs, a decrease in T helper-17+ cells in the cervical lymph nodes and generation of less pathogenic CD4+T cells. B6.nude recipient mice of adoptively transferred CD4+T cells isolated from NK-depleted DS5 donor mice showed significantly less corneal barrier disruption, lower levels of IL-17A, CCL20 and MMP-3 in the cornea epithelia compared to recipients of control CD4+T cells. Conclusions/Significance: Taken together, these results show that the NK IELs are involved in the acute immune response to desiccation-induced dry eye by activating DC, which in turn coordinate generation of the pathogenic Th-17 response. © 2012 Zhang et al.
CITATION STYLE
Zhang, X., Volpe, E. A., Gandhi, N. B., Schaumburg, C. S., Siemasko, K. F., Pangelinan, S. B., … de Paiva, C. S. (2012). NK cells promote Th-17 mediated corneal barrier disruption in dry eye. PLoS ONE, 7(5). https://doi.org/10.1371/journal.pone.0036822
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