Background: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci. Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA. Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value. < 0.001). High genotype concordance between different sources of genomic DNA was obtained. Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B. © 2011 Elsevier B.V.
CITATION STYLE
Lau, K. C., Lam, C. W., Law, C. Y., Lai, S. T., Tsang, T. Y., Siu, C. W. K., … Chan, Y. W. (2011). Non-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: Susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment. Clinica Chimica Acta, 412(11–12), 952–957. https://doi.org/10.1016/j.cca.2011.01.030
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