A special low-molecular-weight peptide named Gt factor, was isolated and purified from the extracellular culture of brown-rot fungi Gloeophyllum trabeum via gel filtration chromatography and HPLC. It has been shown to reduce Fe3+ to Fe2+. Electron paramagnetic resonance (EPR) spectroscopy revealed Gt factor was able to drive H2O2 generation via a superoxide anion O2.- intermediate and mediate the formation of hydroxyl radical HO. in the presence of O2. All the results indicated that Gt factor could oxidize the cellulose, disrupt the inter- and intrahydrogen bonds in cellulose chains by a HO.-involved mechanism. This resulted in depolymerization of the cellulose, which made it accessible for further enzymatic hydrolysis.
CITATION STYLE
Wang, W., & Gao, P. (2002). A peptide-mediated and hydroxyl radical HO.-involved oxidative degradation of cellulose by brown-rot fungi. Biodegradation, 13(6), 383–394. https://doi.org/10.1023/A:1022848414100
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