Production of fluorous-based microarrays with uncharged carbohydrates

Abstract

Fluorous-based carbohydrate microarrays provide an alternative to traditional covalent microarray -platforms for probing protein-carbohydrate- binding interactions. The most studied plant lectin, concanavalin A (ConA), is known to bind to terminally α-linked mannose. In the studies presented, the binding of ConA with α-mannose is analyzed using a microarray formed on a fluorous-coated glass slide with the sugar containing a fluorous tag at the anomeric position. © 2012 Springer Science+Business Media, LLC.