Based on the results of carbon source consumption in cell suspension culture of Panax quinquefolium L., 30 g L -1 sucrose was fed into a 5-L stirred tank bioreactor on day 16 of culture to enhance cell density and metabolite production. Using a fed-batch cultivation strategy, polysaccharide production was enhanced to 1.608 g L -1, which was 1.96-fold greater than with batch cultivation. The maximum saponin yield (7.828 mg L -1) was obtained on day 24 and was about 36% higher than the yields obtained using batch cultivation. In a two-stage culture process, a combined treatment with sucrose, lactoalbumin hydrolysate, and methyl jasmonate caused a significant increase in total saponin yield (31.52 mg L -1) in cell cultures after 27 d. This value represents an increase of 4.03-fold compared with the total saponin yield in fed-batch cultivation. The two-stage culture mode provided the best method for the in vitro production of secondary metabolites from P. quinquefolium. © 2011 The Society for In Vitro Biology.
CITATION STYLE
Wang, J., Gao, W. Y., Zhang, J., Zuo, B. M., Zhang, L. M., & Huang, L. Q. (2012). Production of ginsenoside and polysaccharide by two-stage cultivation of Panax quinquefolium L. cells. In Vitro Cellular and Developmental Biology - Plant, 48(1), 107–112. https://doi.org/10.1007/s11627-011-9396-x
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