Rabbit Serum Amyloid Protein A: Expression and Primary Structure deduced from cDNA Sequences

Abstract

Serum amyloid A protein (SAA), the precursor of amyloid protein A (A.A) in deposits of secondary amyloidosis. is an acute phase plasma apolipoprotein produced by hepatocytes. The primary structure of SAA demonstrates high interspecies homology. Several isoforms exist in individual species, probably with different amyloidogenic potential. The nucleotide sequences of two different rabbit scrum amyloid A cDNA clones have been analysed, one (corresponding to SAA1) 569 base pairs (bp) long and the other (corresponding to SAA2) 513 bp long. Their deduced amino acid sequences differ at five amino acid positions, four of which arc located in the NH;‐terminal region of the protein. The deduced amino acid sequence of SAA2 corresponds to rabbit protein AA previously described except for one amino acid in position 22. Eighteen hours after lurpentine stimulation, rabbit SAA m RNA is abundant in liver, while lower levels are present in spleen. None of the other extrahepatic organs studied showed any SAA mRNA expression, A third mRNA species (1.9 kb) hybridizing with a single‐stranded RNA probe transcribed from the rabbit SAA cDNA. was identified. SAA 1 and SAA2 mRNA were found in approximately equal amounts in turpentine‐stimulated rabbit liver, but seem to be coordinately decreased after repeated inflammatory stimulation. Copyright © 1991, Wiley Blackwell. All rights reserved