Abstract
Recombinant expression of proteins and their complexes is the routine laboratory procedure to generate pure reagents for biochemical and structural studies. Here we present the standard procedure developed in our lab for the production of milligram quantities of stoichiometric SUN–KASH complexes. The protocol was specifically developed for the purification of the periplasmic portion of LINC complexes.
Author supplied keywords
Cite
CITATION STYLE
APA
Cruz, V. E., & Schwartz, T. U. (2018). Recombinant Purification of the Periplasmic Portion of the LINC Complex. In Methods in Molecular Biology (Vol. 1840, pp. 17–23). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8691-0_2
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
Already have an account? Sign in
Sign up for free