Recovery of protein from reverse micelles through gas hydrate formation

Abstract

The recovery of cytochrome c and ribonuclease A from di-2-ethylhexyI sodium sulfosuccinate (AOT) reverse micelles have been examined by the gas hydrate formation. The recovery of cytochrome c depended upon the kind of gas and the water content (W0 =[H2O]/[AOT]) of reverse micellar solution containing cytochrome c prepared. Recoveries of cytochrome c and ribonuclease A were more than 80%, when 1,1,1,2-tetrafluoroethane (TFE) was used as a hydrating gas. The activity of cytochrome c recovered from reverse micelles was maintained perfectly. © 1999 Kluwer Academic Publishers.