Mapping of transcribed mRNA and protein coding sequences is the initial step in functional characterization of genomic sequences. There are several techniques to identify transcribed sequences; however, Northern blot analysis remains a standard method for detection and quantification of mRNA levels despite the relatively recent availability of other methods, such as RT-PCR (reverse transcriptase polymerase chain reaction), which are also sensitive, accurate, cost-effective, and simple to use. Northern blot analysis provides a direct relative comparison of message abundance between samples on a single membrane. It is also the preferred method for determining transcript size and detecting alternatively spliced isoforms. Other methods utilizing RT-PCR allow detection and cloning of isolated complementary DNA (cDNA) domains and ends from cellular RNA or commercially available cDNA libraries. © 2011 Springer Science+Business Media, LLC.
CITATION STYLE
Alvarez, M. L., & Nourbakhsh, M. (2011). RNA mapping protocols: Northern blot and amplification of cDNA ends. Methods in Molecular Biology, 700, 199–220. https://doi.org/10.1007/978-1-61737-954-3_14
Mendeley helps you to discover research relevant for your work.