RNA-Seq: a revolutionary tool for transcriptomics.

RNA-Seq: a revolutionary tool for transcriptomics.

by Zhong Wang, Mark Gerstein, Michael Snyder

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Nature Reviews Genetics (2009)

Volume: 10, Issue: 1, Publisher: Nature Publishing Group, Pages: 57-63

PubMed: 19015660

Available from www.pubmedcentral.nih.gov

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Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

Author-supplied keywords

animals base sequence chromosome mapping exons gene expression profiling gene expression profiling methods genetic humans models molecular sequence data rna rna analysis rna methods sequence analysis transcription

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