Rough Set Analysis of Microrna: Mrna Binding Pattern

Abstract

MicroRNAs(miRNAs) are small noncoding RNAs that silence gene expression by base pairing to mRNAs. The functional characterization of miR- NAs relies heavily on the identi¯cation of their target mRNAs. Although some targets of a handful of miRNAs are known, most of the mRNAs that miRNAs regulate are unknown. To detect miRNAs and their target mRNAs base-pairing pattern in a direct and convenient way, we try to produce binding pattern rules based on two measures of base pairing probability with rough set package ROSETTA. The two measures are e®ective in identify miRNA:mRNA binding pattern and using them we con¯rm that binding patterns with position other than 2..8 are all unreliable in vertebrate miRNAs.