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A set of compatible tac promoter expression vectors

by Derek M. Dykxhoorn, Rebecca St. Pierre, Thomas Linn
Gene ()

Abstract

A series of expression vectors have been developed which all contain an identical expression cassette comprised of the lacI(q) gene, the tac promoter, a multiple cloning site (MCS) and a downstream transcriptional terminator. This cassette has been inserted into four distinct plasmid backbones, each of which is from a separate incompatibility group and carries a different drug resistance gene. Therefore, different combinations of these expression plasmids can be stably maintained together.

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