Structural Model of biochemical network of Zymomonas mobilis adaptation for glycerol conversion into bioethanol

Abstract

One of the biodiesel production problems is a significant quantity (about 10%) of by-product - glycerol occurrence. This problem is offered to solve by adaptation of bacteria Zymomonas mobilis, which is notable for ethanol production facilities. To be able to process glycerine into ethanol using Z. mobilis bacteria, the bacteria must be modified, because its natural form cannot process glycerine. At the same time computer modelling analysis is required, to assess specific modification affectivity in interconnection with other processes in bacteria. The computer model, which describes two genes of - bacteria E. Coli GlpF and GlpK insertions and expressions in bacteria Zymamonas mobilis. These gene-coded proteins ensure glycerol transport - in bacteria and conversion to substances, which bacteria can process to ethanol. Biochemical reactions and process regulation network is too complicated, to be able to predict system's respond without extensive computer modelling by changing any of its components. The model describes conversion of glycerol into bioethanol in Z. mobilis bacterial cell. First phase of model creation is creation of structure model based on biochemical reactions. On the second phase of model creation were identified kinetic parameters which are available in literature. Using the databases KEGG, SABIO-RK, BRENDA, where defined reactants, kinetic parameters and reaction equation types. Computer model of Z. mobilis biochemical network was created using computer software CellDesigner (4.1. beta).