A study on the relationship between human papilloma virus (HPV) and cervical cancer

Abstract

Samples of cervical tissues of patients from Shandong province were detected by using Southern blot hybridization technique of nucleic acid with the probe of (alpha-32P)-dCTP HPV16 DNA fragment cleaving with BamHI. The total positive rate of HPV16 DNA related sequences in 116 cases with cervical cancer was 50.9%, and much higher than that in 36 cases with non-cervical disease (5.6%). There is a strong correlation between the HPV and cervical cancer (OR = 17.60, P less than 0.001). It was showed that there was no association between the positive rate of HPV 16 DNA related sequences and age, clinical period and local appearance in cases with cervical cancer. This was the first paper described that HPV 33 DNA sequences were found in four cervical carcinoma samples, and HPV 31 DNA sequence in one cervical carcinoma sample under low stringent conditions of hybridization in Shandong province.