ESCRT (Endosomal Sorting Complex Required for Transport) proteins are required for the sorting of biosynthetic and endocytic proteins at multivesicular bodies (MVBs). Here, I describe an assay to evaluate conservation of endosomal sorting functions of plant ESCRT proteins by trans-species complementation analysis. The assay is based on the imaging of a fluorescent biosynthetic MVB cargo, the carboxypeptidase S (CPS) fused to the green fluorescent protein GFP, in yeast ESCRT mutants expressing putative plant orthologues of the missing yeast ESCRT components. © 2014 Springer Science+Business Media New York.
CITATION STYLE
Reyes, F. C. (2014). Trans-species complementation analysis to study function conservation of plant endosomal sorting complex required for transport (ESCRT) proteins. Methods in Molecular Biology, 1209, 143–148. https://doi.org/10.1007/978-1-4939-1420-3_11
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