We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
CITATION STYLE
Bowers, J., Mitchell, J., Beer, E., Buzby, P. R., Causey, M., Efcavitch, J. W., … Thompson, J. F. (2009). Virtual terminator nucleotides for next-generation DNA sequencing. Nature Methods, 6(8), 593–595. https://doi.org/10.1038/nmeth.1354
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