In vitro antagonistic activity of soil actinobacteria against multi drug resistant bacteria

Abstract

The purpose of this study was to explore the antimicrobial properties of actinobacteria isolated from the soil samples of Ranipet, Vellore, Tamil Nadu, India. A total of 13 actinobacteria were isolated and screened for antagonistic property against four multi drug resistant (MDR) bacteria included Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Non fermenting gram negative bacilli. Among the 13 isolates, two (AR7 and AR12) were found to be effective against K. pneumoniae and E. coli in primary screening (cross streak method), further the potential isolates were secondary screened by agar well diffusion method, only one isolates (AR 12) was found to be effective against K. pneumoniae and E. coli. Further AR 12 isolate (potential strain) was characterized by morphological test, biochemical test and Nonomura key. Actinobacteria isolate (AR12) identified as Streptomyces gougeroti. This study also reports the antibiogram of the MDR strains used in this study.