In vitro antioxidant activities of methanol and aqueous extract of annona squamosa (L.) fruit pulp

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Abstract

The present study evaluated the antioxidant activity of the fruit of Annona squamosa by means of invitro studies involving two different solvent extracts: methanol and aqueous. The antioxidant properties of the extract were determined by scavenging 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), lipid peroxidation (LPO), nitric oxide (NO), superoxide anion (O2-), hydroxyl radical (OH•), reducing power and total antioxidant. The results showed that, compared to aqueous extract, a methanolic fruit extract of A squamosa has a higher percentage of inhibition of DPPH radical scavenging activity (97.99%), LPO (94.15%), NO scavenging activity (70.96%), O2- scavenging activity and OH• scavenging activity (78.68% and 85.25%, respectively), total antioxidant activity (206μg α-tocopherol/g) and reducing power (56.0μg of ascorbic acid/g). The results obtained in the invitro models clearly suggest that methanol extract has higher antioxidant activity than the aqueous extract due to a higher presence of phenolic and flavonodal constituents in the methanol extract. © 2013, International Pharmacopuncture Institute.

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Nandhakumar, E., & Indumathi, P. (2013). In vitro antioxidant activities of methanol and aqueous extract of annona squamosa (L.) fruit pulp. JAMS Journal of Acupuncture and Meridian Studies, 6(3), 142–148. https://doi.org/10.1016/j.jams.2012.09.002

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