Research Technician - Immunology and Immunotherapy - 54778 - Band 500

Location
Birmingham
Salary
Competitive
Posted
Jul 04, 2019
Closes
Jul 17, 2019
Ref
19000084
Contract Type
Full Time
Job Type
Technican
Position Details

Institute of Immunology and Immunotherapy

University of Birmingham, Edgbaston, Birmingham UK

Band 500

Starting salary is normally in the range £22,722 to £24,411. With potential progression once in post to £28,259 a year.

FTC 6 months

Closing Date : 16 July 2019

Role summary

Cancer Immunology in the Institute of Immunology and Immunotherapy comprises several research groups with an international reputation in the molecular basis of cancer, with expertise in immunology, gene therapy, molecular virology, viral immunology, cell signalling, immunotherapy and clinical trials. This research technician post is funded by the Cell and Gene Therapy Catapult (UK) for 5 months. The post holder will make a significant contribution to the research group led by Dr Steve Lee by helping to develop immunotherapeutic strategies against cancer, principally using genetic modification of T lymphocytes to target them to markers selectively expressed on the tumour vasculature. The project will entail working closely with other members of the lab and collaborators to develop methods and to deliver high quality research using a variety of techniques. The post-holder will also be responsible for taking a lead role in the day to day running of the lab, including the safety and maintenance of equipment and stocks within the lab.

Main duties

Full-time research - 75%

Working under the direction of Dr Lee, to undertake experiments contributing to the objectives set out in the programme of preclinical work funded by the Cell and Gene Therapy Catapult (UK) exploring the safety and efficacy of a T cell based therapy targeting CLEC14A and leading to a phase I clinical trial.
• Work may include immunohistology, molecular cloning, flow cytometry, in vitro assays of T cell function (cytotoxicity, cytokine release and proliferation). In vivo studies will explore the safety, vascular-targeting and anti-tumour effects of selected chimeric antigen receptors (CARs) using mouse tumour models (which selectively overexpresses the target antigen, CLEC14A, in the tumour vasculature).
• Additional work will include cell culture, growing up and characterising plasmid constructs.
• The post holder is also required to keep accurate records of all experiments
• Evaluate and interpret results
• Modify experiments and suggest new experimental approaches as appropriate.

Technical support for the group - 10%

Overall responsibility for the organisation and smooth running of the laboratory and for ensuring adherence to current regulations.
• Maintenance/cleaning of laboratory equipment and facilities.
• Updating and maintaining databases of cells, cell lines and reagents, including antibodies.
• Ensuring the laboratory is adequately stocked with reagents.

Supervision of staff (students and technicians) - 5%

• Training/supervision of new research staff ensuring staff are fully aware of current safety regulations.

Management & Safety - 5%

• Duties to include writing SOPs of experimental methods, risk assessments, advising group members of safety issues and responsibilities.
• Representing the laboratory on local Safety groups and reporting back to the lab any matters arising from the Panel meetings.
• Shared responsibility for the safe handling of chemicals and other possible laboratory hazards, and ensuring staff are fully aware of current safety regulations.

Group participation - 5%

• Attendance and contribution to laboratory meetings and journal clubs

Examples of typical problems that the post-holder needs to solve/ typical decisions the post-holder is required to make.

... without reference to others

• Autonomous prioritisation of work on a daily/weekly basis to optimise time and meet scientific deadlines.
• Select appropriate experimental protocols/techniques and choice of controls as required etc.
• Modifying experimental approaches/protocols to overcome technical problems.
• Routine operation and maintenance of laboratory equipment
• Ordering/replacing consumable reagents and selecting appropriate suppliers of equipment and laboratory reagents.
• Must be able to show evidence of ability to work independently and meet personal deadlines

... after consulting others

• Active contribution to the development of new processes for the group
• Resolution of difficulties in research problems
• Minor equipment/reagent purchase.
• Maintenance of pertinent COSHH and risk assessment documentation
• Direction of future research projects

... or that would be referred to someone else

• Establishing long-term objectives and new research priorities
• Suggesting improvements to research protocols and experimental approaches
• Contributing to the group publication output.
• Major equipment purchase

Knowledge, Skills, Qualifications and Experience Required

BTEC (Higher), BSc or equivalent in a Biological Science
In-depth practical knowledge of technical and scientific methods in a wide range of experimental methodologies including flow cytometry, immunohistology and/or cell culture. Molecular cloning and experience with viral vectors for gene transfer would also be an advantage.
Knowledge of the use of chemical substances, radioactivity and safe laboratory practice
Must be able to use initiative, work independently and communicate scientific results to the other members of the group
Highly organised, conscientious, punctual and shows attention to detail.
Ability to acquire techniques quickly and accurately

Other members of the Lee lab - Purpose/skills required

To coordinate effective working within the laboratory on closely related projects. Skills required = good communication, attention to detail and team work.

Collaborators on the grant - Purpose/skills required

To organise shipments of reagents between collaborating laboratories. Skills required = good communication, attention to detail and team work.

Informal Enquries to Dr Steve Lee s.p.lee@bham.ac.uk

http://www.download.bham.ac.uk/vacancies/jd/MDSFPs.pdf