Application of click chemistry to the production of DNA microarrays

Abstract

The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5′-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments. © 2012 The Royal Society of Chemistry.