Skip to content

Characterization of a Listeria monocytogenes Protein Interfering with Rab5a

by Carmen Alvarez-Dominguez, Fidel Madrazo-Toca, Lorena Fernandez-Prieto, Joël Vandekerckhove, Eduardo Pareja, Raquel Tobes, Maria Teresa Gomez-Lopez, Elida Del Cerro-Vadillo, Manuel Fresno, Francisco Leyva-Cobián, Eugenio Carrasco-Marín show all authors
Traffic ()
Get full text at journal


Listeria monocytogenes (LM) phagocytic strategy implies recruitment and inhibition of Rab5a. Here, we identify a Listeria protein that binds to Rab5a and is responsible for Rab5a recruitment to phagosomes and impairment of the GDP/GTP exchange activity. This protein was identified as a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Listeria (p40 protein, Lmo 2459). The p40 protein was found within the phagosomal membrane. Analysis of the sequence of LM p40 protein revealed two enzymatic domains: the nicotinamide adenine dinucleotide (NAD)-binding domain at the N-terminal and the C-terminal glycolytic domain. The putative ADP-ribosylating ability of this Listeria protein located in the N-terminal domain was examined and showed some similarities to the activity and Rab5a inhibition exerted by Pseudomonas aeruginosa ExoS onto endosome-endosome fusion. Listeria p40 caused Rab5a-specific ADP ribosylation and blocked Rab5a-exchange factor (Vps9) and GDI interaction and function, explaining the inhibition observed in Rab5a-mediated phagosome-endosome fusion. Meanwhile, ExoS impaired Rab5-early endosomal antigen 1 (EEA1) interaction and showed a wider Rab specificity. Listeria GAPDH might be the first intracellular gram-positive enzyme targeted to Rab proteins with ADP-ribosylating ability and a putative novel virulence factor.

Cite this document (BETA)

Authors on Mendeley

Readership Statistics

30 Readers on Mendeley
by Discipline
73% Agricultural and Biological Sciences
13% Medicine and Dentistry
7% Immunology and Microbiology
by Academic Status
37% Researcher
23% Student > Ph. D. Student
13% Student > Master
by Country
3% Spain

Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Sign up & Download

Already have an account? Sign in