We present a cross-species chemogenomic screening platform using libraries of haploid deletion mutants from two yeast species, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We screened a set of compounds of known and unknown mode of action (MoA) and derived quantitative drug scores (or D-scores), identifying mutants that are either sensitive or resistant to particular compounds. We found that compoundĝ€"functional module relationships are more conserved than individual compoundĝ€"gene interactions between these two species. Furthermore, we observed that combining data from both species allows for more accurate prediction of MoA. Finally, using this platform, we identified a novel small molecule that acts as a DNA damaging agent and demonstrate that its MoA is conserved in human cells. © 2010 EMBO and Macmillan Publishers Limited All rights reserved.
CITATION STYLE
Kapitzky, L., Beltrao, P., Berens, T. J., Gassner, N., Zhou, C., Wüster, A., … Krogan, N. J. (2010). Cross-species chemogenomic profiling reveals evolutionarily conserved drug mode of action. Molecular Systems Biology, 6. https://doi.org/10.1038/msb.2010.107
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