Influenza virus M1 protein binds to RNA through its nuclear localization signal

by Christine Elster, Kjeld Larsen, Jean Gagnon, R. W H Ruigrok, Florence Baudin
Journal of General Virology ()


The RNA-binding activity of influenza A virus M1 protein was studied by cross-linking the protein to viral RNA followed by sequence analysis of the oligoribonucleotide bound to the protein as well as sequence analysis of the M1 peptide bound to the RNA. M1 was found to bind to RNA without any RNA sequence specificity, as verified in a series of filter-binding experiments using a large variety of nucleic acids including DNA. The peptide sequence that bound to the RNA was the RKLKR nuclear localization signal of M1. Site-specific mutagenesis of recombinant M1 showed that most of the basic residues in that region had to be mutated in order to inhibit RNA-binding. We also constructed an M1 mutant that no longer bound to RNA but which was still able to inhibit the in vitro transcription activity of isolated viral ribonucleoprotein, albeit to a lower extent. Mutation of the zinc-binding sequence had no effect on RNA-binding or transcription-inhibition activity.

Cite this document (BETA)

Authors on Mendeley

  1. Jean Gagnon
    Researcher (at an Academic Institution)
    Grenoble, France

Readership Statistics

24 Readers on Mendeley
by Discipline
by Academic Status
29% Ph.D. Student
21% Post Doc
8% Other Professional
by Country
8% United States
4% France


Sign up today - FREE

Mendeley saves you time finding and organizing research. Learn more

  • All your research in one place
  • Add and import papers easily
  • Access it anywhere, anytime

Start using Mendeley in seconds!

Sign up & Download

Already have an account? Sign in