The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38-46), Dicerca aenea validiuscula Semenov, 1895-2n=20 (18+Xyp) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998-2n=16 (14+Xyp) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A3 (CMA3) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA3 staining. © Gayane Karagyan et al.
CITATION STYLE
Karagyan, G., Lachowska, D., & Kalashian, M. (2012). Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, agnor-banding and CMA3/DAPI staining. Comparative Cytogenetics, 6(2), 183–197. https://doi.org/10.3897/CompCytogen.v6i2.2950
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