A new approach to assess the total antioxidant capacity using the TEAC assay

by M.J.T.J. Arts, J.S. Dallinga, H.-P. Voss, G.R.M.M. Haenen, A. Bast
Food Chemistry ()


The trolox equivalent antioxidant capacity (TEAC) assay is a popular method for assessing the capacity of a compound to scavenge ABTS radicals (ABTS). Under the conditions in which the assay is performed, the reaction between most antioxidants and ABTS does not reach completion within the time span applied. This leads to an underestimation of the TEAC of these antioxidants. In the present study, incubations with different concentrations of ABTS and a fixed concentration of antioxidant were performed. The decrease in ABTS concentration in 6 min was plotted against the initial concentration of ABTS and fitted by an exponential function. Extrapolation of the fit to an infinite excess of ABTS gives the maximal concentration of ABTS that can be scavenged by the antioxidant at the concentration employed. This can be used to determine the actual TEAC of antioxidants, i.e. the total an- tioxidant capacity.

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