Anti-inflammatory effect of birsonimadiol from seeds of Byrsonima crassifolia

Abstract

The new compound identified as 16α,23α-dihydroxy-3β,28β,30α-triacetoxy-olean-12-ene, named birsonimadiol (BIR) was isolated from Byrsonima crassifolia seeds using activity-guided fractionation and structural elucidation was achieved based on extensive analysis of spectroscopic data. Effects of BIR on acute and chronic phases of inflamation were studied in edema induced using formaldehyde, 12-O-tetradecanoylphorbol-acetate (TPA)-induced ear edema (meloperoxidase activity), and histamine, carrageenan, cotton pellet granuloma, and adjuvant-induced arthritis. Anti-inflammatory activities of BIR were indicated based on reduction of edema levels induced in models of inflammation. Anti-inflammatory activities were also investigated in murine macrophage RAW264.7 cells. BIR suppressed production of nitric oxide (NO) and prostaglandin E2 (PGE2), decreased gene expression of cyclooxygenase-2 (COX-2), inhibited tumor necrosis factor (TNF)-α, and protein secretion of interleukin IL-6. Triterpene was an effective topical anti-inflammatory agent in experimental models of acute and chronic dermatitis and can be used in inflammatory disorders.