Isolation, Characterization of Phosphatase Producing Bacteria and Fungi-Their Effect on Enhancing the Growth of Vigna radiata

Abstract

Background: Phosphate solubilizing microorganisms (PSM) are considered as most important traits associated with plant phosphate nutrition and growth. The challenge of study include identification of potent microorganisms that can be used as phosphate solubilizer. Methods: In this study during 2017-2018 study nine isolates were isolated from soil, dung, lakes, five bacterial isolates (A, B, C, D, E) identified as, Bacteroides sp, four fungal isolates Aspergillus sp (F1) and Mucor sp (F2, F3, F4). Their effect on plant (Vigna radiata) growth was studied. Maximum phosphatase production was observed by B (266 µg/ml) and F2 (297.5 µg/ml) isolates and also phosphate by B (10.6 µg/ml) and F2 (42.6 µg/ml). Increase in shoot length and number of roots was observed in the presence of isolates. Result: The contribution of study indicate, phosphate solubilizing isolates, have probable application as biofertilizer and may help in future agricultural needs.