ANTIBACTERIAL AND ANTICANCER ACTIVITY OF PROTEIN SPONGES COLLECTED FROM THE WATERS OF KAPOPOSANG ISLAND OF SOUTH SULAWESI, INDONESIA

Abstract

This study reported the protein bioactivity of two sponges Haliclona fascigera and Clathria reinwardti, collected from the waters of Kapoposang island of South Sulawesi in the search for potential new drugs. Protein extracts were subjected to antibacterial activity test and anticancer activity screening. Isolation of the protein fraction was carried out using a polar solvent (Tris-HCl buffer 0.1 M). Purification of protein was done by fractionation method using ammonium sulphate followed by dialysis and finally determination of protein content was carried out by Lowry method. An Antibacterial activity tests includes diffusion method using paper disc and anticancer activity screening with Brine Shrimp Lethality Test (BSLT). The results showed that protein from sponge Clathria reinwardtii (0-20% fraction) and sponge Haliclona fascigera (20-40% fraction) were effective in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria, but not effectively as new antibacterial agents. The most toxic anticancer activity screening for shrimp larvae A. salina Leach was seen in the protein fraction of sponge Haliclona fascigera (20-40% fraction) with LC50 value = 0.013 mug/mL.Copyright © 2019 International Research Journal of Pharmacy. All Rights Reserved.