Characterization of a mouse DNA clone containing an immunoglobulin variable region gene

Abstract

A 4.8 kilobase mouse embryo DNA fragment was inserted into a phage λ genome and was subsequently characterized by electron microscopy, restriction enzyme mapping and partial DNA sequencing. This fragment contains a 400 base sequence which is homologous to that of an immunoglobulin light λ chain mRNA which spans 3.3 to 3.7 kilobases from one end of the fragment. Restriction enzyme mapping as well as partial nucleotide sequencing of the 3′ terminal of the homology region confirm the previous conclusion [Tonegawa, Brack, Hozumi and Schuller, Proc. Natl. Acad. Sci. USA. 74, 3518-3522 (1977)] that the cloned DNA fragment contains a Vλ gene sequence which is separate from any Cλ sequence. © 1978 Information Retrieval Limited.