Cytotoxic and antimicrobial activities of two new triterpenoids from the peels of local egyptian malus domestica l.

Abstract

The local Egyptian peels of Malus domestica was extracted successively with absolute ethanol and partitioned sequentially with n-hexane, chloroform and methanol. Friedelin (1) was isolated from the n-hexane fraction, and betulinic acid (2) from the chloroform fraction. The new compounds 2α-hydorxy-3-oxolup- 12(13), 20(29)-dien-27, 28-dioic acid (3) and 2α, 27-dihydorxy-3-oxo-lup-12(13), 20(29)-dien-28-oic acid (4) were isolated from the methanol fraction and elucidated by spectroscopic data interpretation. Friedelin (l) and betulinic acid (2) are known compounds that are newly identified from peels of Malus domestica. The compounds 3 &4 are appreciably active against Gram-positive species and Gram-negative species, associated with week anti-fungal activity. Among them, compounds (3) and (4) exhibited cytotoxic activity against the breast carcinoma and human colon carcinoma cells, with (4) being slightly most potent toward to the breast carcinoma while, (3) being slightly most potent toward to the human colon carcinoma cells .