Sequence analysis of flavanone 3-hydroxylase and dihydroflavonol 4-reductase genes in wheat with nonstandard coloured caryopses

Abstract

The DNA sequences of chosen structural genes, flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR), encoding key enzymes from the flavonoid biosynthetic pathway, were studied in this paper. Sequences were gained using different approaches, i.e. direct sequencing from the PCR product for F3H and a cloning strategy for DFR. Five bread wheat (Triticum aestivum L.) genotypes with nonstandard coloured caryopses, purple, blue, and white, were used as plant material. The sequence variability was observed among tested genotypes.