Laser micromanipulation in the mouse embryo: A novel approach to zona drilling

0Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

Abstract

Objective: To introduce the use of excimer lasers for penetration of the zona pellucida for micromanipulation purposes. Design: Cryopreserved two-cell mouse embryos were thawed and exposed to the 248-nm line of a krypton fluoride excimer laser (Lambda Physik EMG 202, Goettingen, Germany) creating a 2 to 4-μm opening in the zona pellucida. Setting: The Laser Ablation Laboratory at DuPont and the in Vitro Fertilization Laboratory at The Medical Center. Interventions: The embryos were exposed in either phosphate-buffered solution (PBS) or modified human tubal fluid (HTF) with the laser power varying from 1 to 2 J/cm2 and cultured in Ham's F-10 medium (GIBCO, Grand Island, NY) with 0.4% bovine serum albumin. Main Outcome Measures: The outcome of each experiment was measured by blastocyst formation of laser- exposed embryos as compared with a set of unexposed control embryos handled in a similar fashion. Results: Successful laser penetration of the zona pellucida was achieved using the 248-nm line of a krypton fluoride excimer laser. A higher blastocyst formation was found for embryos exposed in PBS. The higher optical absorption of the modified HTF partially inhibited embryo development. The blastocyst statistics increased 2.5-fold times by reducing the exposure of the embryos to ablation by-products. Conclusions: The use of a krypton fluoride excimer laser was introduced as a new method to open the zona pellucida of two-cell mouse embryos without interrupting blastocyst formation.

Author supplied keywords

Cite

CITATION STYLE

APA

Blanchet, G. B., Russell, J. B., Fincher, C. R., & Portmann, M. (1992). Laser micromanipulation in the mouse embryo: A novel approach to zona drilling. Fertility and Sterility, 57(6), 1337–1341. https://doi.org/10.1016/S0015-0282(16)55097-3

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free