Transcript profile in vegetable soybean roots reveals potential gene patterns regulating k uptake efficiency

Abstract

Significant differences have been reported in root K+ uptake between high potassium efficiency (HKE) and low potassium efficiency (LKE) in vegetable soybean genotypes. The ideal morphological and physiological characteristics of HKE have been defined. However, the mechanism by which HKE vegetable soybean genotypes efficiently uptake K remains unclear. By using representative materials, this study investigated the responses of root development to low K (LK) stress, and identified and assessed the key genes affecting high-efficiency K uptake between HKE and LKE vegetable soybean roots. The root growth of LKE was significantly inhibited under the LK condition. Compared with LKE, HKE had more lateral roots in both LK and CK (control) conditions. Lateral root of HKE was more preferentially responsive to exogenous IAA, with a wider response threshold to IAA concentration (from 0.1 to 1 µM). Transcriptome analysis revealed that LK induced transport-related genes up-regulated in HKE compared with LKE. In HKE, homologous genes of a K channel encoding gene potassium channel AKT1 (AKT1) and a K transporter gene high-affinity K+ transporter 5 (HAK5) were both highly expressed under the LK stress. Additionally, genes related to plant hormones signal transductions were also identified differentially expressed between the two genotypes. Plant hormone signaling involved in root morphological regulation pathways may play significant roles in improving the efficiency of vegetable soybean K+ uptake. A diagram showing possible molecular mechanisms in regulating root high-efficiency uptake K+ in vegetable soybean is proposed.