The K99 antigen of E. coli can be detected more readily in cultures grown on Minca medium at 37°C for 6 to 8 hr or grown on Minca plus 1% IsoVitaleX for 20 to 24 hr.
CITATION STYLE
Guinee, P. A. M., Veldkamp, J., & Jansen, W. H. (1977). Improved Minca medium for the detection of K99 antigen in calf enterotoxigenic strains of Escherichia coli. Infection and Immunity, 15(2), 676–678. https://doi.org/10.1128/iai.15.2.676-678.1977
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