Optimized Configuration of Fixed-Tip Robotic Liquid-Handling Stations for the Elimination of Biological Sample Cross-Contamination

19Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Robotic liquid-handling stations (RLHSs) are the mainstay of high-throughput biomedical/forensic DNA sample processing facilities. These liquid-handling systems can be alternatively tooled with either disposable or fixed-tip pipetting heads. The use of disposable tips is often perceived as the best tip configuration to eliminate cross-contamination between biological samples processed on liquid-handling stations. However, this suppression can be effectively achieved on instruments equipped with fixed tips with optimally designed tip wash station (WS) configurations. Fixed-tip instruments offer many significant sample processing advantages with respect to precision, pipetting of liquids that may contain aggregates, and operational cost. This report discusses how cross-contamination suppression was achieved for the reliable processing of forensic casework samples on specially configured fixed-tip TECAN Genesis RSP/Freedom EVO RLHSs. A critical analysis of the major components involved in tip washing, as well as the specifications of a redesigned tip-washing routine that increases wash effectiveness and significantly reduces processing time and cost is also presented. © 2007, Society for Laboratory Automation and Screening. All rights reserved.

Cite

CITATION STYLE

APA

Frégeau, C. J., Yensen, C., Elliott, J., & Fourney, R. M. (2007). Optimized Configuration of Fixed-Tip Robotic Liquid-Handling Stations for the Elimination of Biological Sample Cross-Contamination. Journal of Laboratory Automation, 12(6), 339–354. https://doi.org/10.1016/j.jala.2007.08.001

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free