A novel method for pulmonary research: Assessment of bioenergetic function at the air-liquid interface

0Citations
Citations of this article
46Readers
Mendeley users who have this article in their library.

Abstract

Air-liquid interface cell culture is an organotypic model for study of differentiated functional airway epithelium in vitro. Dysregulation of cellular energy metabolism and mitochondrial function have been suggested to contribute to airway diseases. However, there is currently no established method to determine oxygen consumption and glycolysis in airway epithelium in air-liquid interface. In order to study metabolism in differentiated airway epithelial cells, we engineered an insert for the Seahorse XF24 Analyzer that enabled the measure of respiration by oxygen consumption rate (OCR) and glycolysis by extracellular acidification rate (ECAR). Oxidative metabolism and glycolysis in airway epithelial cells cultured on the inserts were successfully measured. The inserts did not affect the measures of OCR or ECAR. Cells under media with apical and basolateral feeding had less oxidative metabolism as compared to cells on the inserts at air-interface with basolateral feeding. The design of inserts that can be used in the measure of bioenergetics in small numbers of cells in an organotypic state may be useful for evaluation of new drugs and metabolic mechanisms that underlie airway diseases. © 2014 The Authors.

Cite

CITATION STYLE

APA

Xu, W., Janocha, A. J., Leahy, R. A., Klatte, R., Dudzinski, D., Mavrakis, L. A., … Erzurum, S. C. (2014). A novel method for pulmonary research: Assessment of bioenergetic function at the air-liquid interface. Redox Biology, 2(1), 513–519. https://doi.org/10.1016/j.redox.2014.01.004

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free