Golgi Membranes Remain Segregated from the Endoplasmic Reticulum during Mitosis in Mammalian Cells

70Citations
Citations of this article
46Readers
Mendeley users who have this article in their library.

Abstract

What happens to organelles during mitosis, and how they are apportioned to each of the daughter cells, is not completely clear. We have devised a procedure to address whether Golgi membranes fuse with the Endoplasmic Reticulum (ER) during mitosis via the detection of interactions between ER and Golgi proteins. This procedure involves coexpressing an FKBP-tagged Golgi enzyme with an ER-retained protein fused to FRAP in COS cells. Since treatment with rapamycin induces a tight association between FKBP and FRAP, one would expect rapamycin to trap the FKBP-fused Golgi protein in the ER if it ever visits the ER during mitosis. However, after the doubly transfected cells progress through mitosis in the presence of rapamycin, we find the Golgi protein in the newly formed Golgi stacks and not in the ER. Based on these results, we conclude that Golgi membranes remain separate from the ER during mitosis in mammalian cells.

Cite

CITATION STYLE

APA

Pecot, M. Y., & Malhotra, V. (2004). Golgi Membranes Remain Segregated from the Endoplasmic Reticulum during Mitosis in Mammalian Cells. Cell, 116(1), 99–107. https://doi.org/10.1016/S0092-8674(03)01068-7

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free