The effect of colchicine on the size and bioactive compound of microalgae Spirulina platensis

Abstract

Polyploidy is one of the techniques used to increase the genetic variant and once used as a breeding method of plant. Colchicine is one of the chemical which apply to produce polyploid organisms, such as plant. This study aimed to determine the effect of colchicine on the size and phycocyanin content of Spirulinaplatensis.Research was used six treatments of colchicine concentration with three replications. S. platensis were immersed in the colchicine solution for 12 hours and were observed for 5 days culture. This research was showed that colchicine concentration of 0.1 % were resulted highest diameter of S. platensis(12.57 μm) while highphycocyanin content obtained by treatment of 0.025 % (0.091 mg/ml).