A Simple and Fast Non-Radioactive Bridging Immunoassay for Insulin Autoantibodies

6Citations
Citations of this article
24Readers
Mendeley users who have this article in their library.

Abstract

Type 1 diabetes (T1D) is an autoimmune disease which results from the destruction of pancreatic beta cells. Autoantibodies directed against islet antigens are valuable diagnostic tools. Insulin autoantibodies (IAAs) are usually the first to appear and also the most difficult to detect amongst the four major islet autoantibodies. A non-radioactive IAA bridging ELISA was developed to this end. In this assay, one site of the IAAs from serum samples is bound to a hapten-labeled insulin (GC300-insulin), which is subsequently captured on anti-GC300 antibody-coated 96-well plates. The other site of the IAAs is bound to biotinylated insulin, allowing the complex to be detected by an enzyme-streptavidin conjugate. In the present study, 50 serum samples from patients with newly diagnosed T1D and 100 control sera from non-diabetic individuals were analyzed with our new assay and the results were correlated with an IAA radioimmunoassay (RIA). Using IAA bridging ELISA, IAAs were detected in 32 out of 50 T1D children, whereas with IAA RIA, 41 out of 50 children with newly diagnosed T1D were scored as positive. In conclusion, the IAA bridging ELISA could serve as an attractive approach for rapid and automated detection of IAAs in T1D patients for diagnostic purposes. © 2013 Kikkas et al.

Cite

CITATION STYLE

APA

Kikkas, I., Mallone, R., Tubiana-Rufi, N., Chevenne, D., Carel, J. C., Créminon, C., … Morel, N. (2013). A Simple and Fast Non-Radioactive Bridging Immunoassay for Insulin Autoantibodies. PLoS ONE, 8(7). https://doi.org/10.1371/journal.pone.0069021

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free