A mimotope of Pre-S2 region of surface antigen of viral hepatitis B screened by phage display

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Abstract

To acquire the phage-displayed mimotopes which mimic the specificity of hepatitis B virus surface antigen (HBsAg), a random peptide library expressing linear peptide with 12 amino acids in length were used to screen with the serum from a hepatitis B virus infected patient in the recovery phase. After 3 rounds of biopanning, the positive phages were confirmed by competitive ELISA using HBsAg/P33. Two phagotopes were identified and one of them was confirmed as mimotope by competition experiment. Based on the mimotpe, a multiple antigenic peptide with four branches was synthesized by solid phase peptide synthesis. The antiginicity and specificity of the synthesized antigen was tested in BALB/c mice compared with the native epitope-based antigen. The results showed that the mimotope-based antigen could evoke higher titer of antibodies with the same specificity of the epitope-based antigen. Those findings indicate mimotopes can be used in antigen and vaccine design.

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Wu, Y. Z., Wan, Y., Li, D. G., Tang, Y., & Zhou, W. (2001). A mimotope of Pre-S2 region of surface antigen of viral hepatitis B screened by phage display. Cell Research, 11(3), 203–208. https://doi.org/10.1038/sj.cr.7290087

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