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Among the laboratory and bioinformatic processing steps for human microbiome studies, a lack of consistency in DNA extraction methodologies is hindering the ability to compare results between studies and sometimes leading to errant conclusions. The purpose of this article is to highlight the issues related to DNA extraction methods and to suggest minimum standard requirements that should be followed to ensure consistency and reproducibility.
Leigh Greathouse, K., Sinha, R., & Vogtmann, E. (2019, October 21). DNA extraction for human microbiome studies: The issue of standardization. Genome Biology. BioMed Central Ltd. https://doi.org/10.1186/s13059-019-1843-8