Chemiluminescence-based detection of peptide activity and peptide-receptor binding in plants

Abstract

In living organisms, physical interaction of ligand molecules with their cognate receptors is an indispensable requirement for the initiation of cellular signaling pathways. To technically prove the biochemical interaction of ligands with their corresponding receptor, a biologically active but labeled peptide is required. Easily scorable bioassays, such as the production of reactive oxygen species, can be used to quantify the activity of a peptide. By using chemiluminescent probes, such as acridinium esters, as conjugates to label peptide ligands of interest, quantitative measurements of ligand-receptor binding can be performed in standard luminometers. Here we describe how this binding approach can be used to reveal peptide ligand-receptor binding in plant material.